Regulatory

Part:BBa_K337057:Design

Designed by: Rebecca Berrens   Group: iGEM10_Heidelberg   (2010-10-26)


synthetic binding site of miR 122 (imperfect) KD: 24%


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

designed to lead to 30% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122.


Source

synthetic

References